To evaluate antihistaminic, antiallergic and mast cell stabilizing effect of phytosterol rich fractions of Leptadenia reticulata (Retz) in various experimental models. In the present study The Unsaponifiable matter (LRUSM) from petroleum extract, acetone (LRA), and methanol (LRM) fraction of the aerial parts of Leptadenia reticulata (Retz) Wight and Arn. (Asclepiadaceae) were studied for Antihistaminic, antiallergic and mast cell stabilizing effect were studied on the Histamine aerosol induced anaphylactic shock induced bronchospasm in guinea pigs. Cows Milk induced eosinophilia in mice, and Clonidine induced mast cell degranulation in rats in the animal model. Treatment with unsaponifiable matter (LRUSM) and LRM treated groups resulted significant protection (P< 0.001) against histamine aerosol-induced bronchospasm in guinea pigs. LRUSM and LRM showed significant Dose dependent (50 &100 mg/kg p.o) decrease in no of eosinophils count and also reduced clonidine induced mast cell degranulation in rats, L. reticulata showed significant decrease in no. of degranulated mast cells count and significant increase in no. of intact mast cells count induced mast cell degranulation in rats. Antihistaminic, antiallegic activity aerial part of Leptadenia reticulata may be possibly due to the membrane stabilising potential, suppression of antibody production and inhibition of antigen induced histamine, suppression of IgE antibody production, due to compound like carbohydrate, glycosides, flavonoids, tannins, phytosterols like �Ÿ-sitosterol, stigmasterol, in LRUSM , Phenolic compounds in acetone(LRA) and methanolic extracts(LRM) of aerial part Leptadenia reticulata(Retz).
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